Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis via Nrf2/NF-κB/NLRP3 axis

Mingxuan Cheng¹, Pu Wang², Di Wu³

¹Department of Prosthodontics, Peking University Hospital of Stomatology First Clinical Division, Beijing 100034, China; ²School of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China; ³Department of Stomatology, Lishui Peopleâ€™s Hospital (The 6th Affiliated Hospital of Wenzhou Medical University), Lishui City, Zhejiang Province 323020, China.

For correspondence:- Di Wu Email: di_w10@163.com Tel:+865782187178

Accepted: 30 October 2022 Published: 30 November 2022

Citation: Cheng M, Wang P, Wu D. Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis via Nrf2/NF-κB/NLRP3 axis. Trop J Pharm Res 2022; 21(11):2303-2308 doi: 10.4314/tjpr.v21i11.5

© 2022 The authors.
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Abstract

Purpose: To investigate the therapeutic role of diosmetin on periodontitis and its possible mechanism of action.

Methods: Lipopolysaccharide (LPS) was used to induce periodontitis in periodontal cells. Cell viability and apoptosis in response to LPS and diosmetin were evaluated using t MTT and TUNEL assays, while oxidative stress and inflammatory responses in LPS-induced periodontitis and diosmetin effects in periodontal cells were determined by enzyme-linked immunosorbent (ELISA) assays. In addition, the roles of diosmetin in pyroptosis and Nrf2/NF-kappa B/NLRP3 pathway were evaluated by immunoblot assay.

Results: Diosmetin increased the viability of LPS-induced periodontal cells (p < 0.01), and also alleviated the oxidative stress of periodontal cells (p < 0.01). It reduced the secretion of proinflammatory factors in periodontal cells and inhibited cell pyroptosis (p < 0.01). Furthermore, it mediated Nrf2/NF-kappa B/NLRP3 pathway (p < 0.01).

Conclusion: Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis through Nrf2/NF-κB/NLRP3 axis. Therefore, it can potentially be used for the management of periodontitis.

Keywords: Purpose: To investigate the therapeutic role of diosmetin on periodontitis and its possible mechanism of action. Methods: Lipopolysaccharide (LPS) was